1.35.4
What is SciFlow? Sign In
Read-only view of a live SciFlow workflow — this is the actual pipeline that produced these results, not a mockup. 16S Amplicon — Human Body-Site Microbiome (SciFlow). Open any stage to inspect its parameters, command, versions, inputs and outputs.
Loading workflow...
Name
Modified
Size

No pipeline outputs

Run stages to generate files here.

No reference papers

This demo has no reference papers attached.

Timeline
May 30, 2026
–
Set end date
Owner Hidden for review Tags
16S amplicon microbiome gut soil
Created May 30, 2026 at 19:50
Which microbes live at four human body sites, and how distinct are those communities? This demo runs the real QIIME 2 Moving Pictures 16S dataset (Caporaso et al. 2011, 34 samples across gut, tongue, left palm, and right palm) through denoising, taxonomy assignment, and community analysis. Key results from this run: PERMANOVA on body_site R²=0.563, p=0.001 — body site explains 56% of community variance and the separation is highly significant. PCoA (Bray–Curtis) captures 50.3% + 21.6% of variance on the first two axes. 42 indicator species identified after BH correction. The gut community separates strongly from oral and skin sites; left and right palm are not distinct from each other, reflecting a shared skin microenvironment. Note on the ordination: NMDS collapses to a near-zero stress solution on this dataset because the body-site groups are so well-separated — the PCoA is the more informative ordination here. Pipeline: DADA2 denoising → QIIME 2 taxonomy (Silva 138) → alpha/beta diversity → PERMANOVA → indicator species (IndVal). Built for microbiome labs who want a defensible, end-to-end 16S profiling and diversity workflow on real, well-characterised human data.