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Read-only view of a live SciFlow workflow — this is the actual pipeline that produced these results, not a mockup. Functional Annotation — E. coli K-12 (SciFlow). Open any stage to inspect its parameters, command, versions, inputs and outputs.
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Created Apr 29, 2026 at 03:12
Take a set of bacterial protein sequences and come back with orthology assignments, domain architectures, GO terms, and KEGG module completeness — all in one pass. Drop in the E. coli K-12 proteome (or your own), and the pipeline tells you what each protein probably is, where its domains came from, which pathways are intact, and which functional categories are over-represented in any subset you care about. Built for microbial genomicists who just finished an assembly, comparative genomics groups annotating new isolates, and anyone who needs a defensible functional summary without stitching five tools together by hand. Homology, orthology, and function are three different things, and the pipeline labels them that way. eggNOG-mapper provides ortholog-based GO and KEGG transfer at controlled evolutionary distance. InterProScan adds domain- and signature-level evidence — Pfam, TIGRFAM, SUPERFAMILY — so you see the actual structural building blocks, not just a top BLAST hit. KEGG module reconstruction reports completeness per pathway with the missing steps named explicitly. The output is a single browsable report with each annotation traceable to the tool and the threshold that produced it.